Serum fingerprint detection based on surface-enhanced Raman scattering
10.7644/j.issn.1674-9960.2016.07.016
- VernacularTitle:基于表面增强拉曼散射的血清指纹谱检测方法研究
- Author:
Jing GUO
;
Zhen RONG
;
Rui XIAO
;
Shengqi WANG
- Publication Type:Journal Article
- Keywords:
spectrum analysis,Raman;
serum dose;
incubation time;
incubation temperature;
treatment of sera
- From:
Military Medical Sciences
2016;40(7):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the experiment conditions of surface-enhanced Raman spectroscopy detection of serum fingerprint spectra.Methods Normal human serum was used as the sample and Ag nanoparticles as the active substrate.The enhanced signals of different optimized experiments were obtained , including serum dose(2.5 to 500 μl), incubation time(10 to 30 minutes) temperature(4℃,room temperature and 37℃),and different treatment(extraction and protein removal).Results and Conclusion Serum doses should not exceed 50μl.The ratio should range from 1∶1 to 5∶1, the incubation time is from 10 to 30 minutes, and the incubation temperature from 4℃ to 37℃.The signals of samples directly mixed with an active substrate are stronger than those of samples which are extracted or protein removed .
