Establishment of ELISA screening method in blood donors with IgA deficiency
10.3969/j.issn.1673-4130.2016.14.020
- VernacularTitle:IgA 缺乏症献血者酶联免疫试验筛查方法的建立
- Author:
Wensheng YUAN
;
Feng YI
- Publication Type:Journal Article
- Keywords:
IgA;
enzyme immunoassay;
IgA deficiency;
blood donor
- From:
International Journal of Laboratory Medicine
2016;37(14):1951-1953
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the enzyme‐linked immuno sorbent assay(ELISA) method for a large‐scale screening of IgA deficiency in blood donors .Methods The indirect ELISA was adopted .The goat anti‐human IgA antibody was coated in microwell plates and labeled by horse radish peroxidase (HRP) as enzyme‐labelled secondary antibody .Results The sensitivity of established ELISA detection method was 0 .1 μg/mL .The intraassay coefficients of variation (CV) for IgA concentrations of 0 .1 ,100 μg/mL were 1 .74% to 3 .49% .The median interassay CV was 3 .48% (range:1 .83% -6 .96% ) .The assay process was 80 min . Conclusion TheELISA detection method is successfully established with high sensitivity ,strong specificity ,timesaving and easy operating and can be used for a large scale screening of Ig A deficiency and establishment of blood donors bank of Ig A deficiency .
