An electrochemical biosensor based on a DNA tetrahedral nanostructure probe for the detection of Ebola virus nucleotide
10.7644/j.issn.1674-9960.2016.07.005
- VernacularTitle:应用DNA四面体探针电化学生物传感器检测埃博拉病毒核酸
- Author:
Jinyan WANG
;
Wen KONG
;
Shibiao DONG
;
Leili JIA
;
Chao LIU
;
Rongzhang HAO
;
Hongbin SONG
- Publication Type:Journal Article
- Keywords:
DNA tetrahedron;
electrochemical biosensor;
Ebola virus
- From:
Military Medical Sciences
2016;40(7):554-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quick electrochemical biosensor for the detection of nucleic acid of Ebola virus . Methods The DNA tetrahedral nanostructure was self-assembled on gold surface by strong Au-S chemical bonds , leaving the target probe at the top .A biotinylated-ssDNA was introduced as the detection probe by specific binding of the captured target sequence , before avidin-horseradish peroxidase ( HRP) was used as a signal amplifier to transduce amperometric sig-nal through interactions with TMB substrate .Results The results indicated that the nucleotide sequence of Ebola virus could be recognized and detected by the sensor .The linear range for the detection of target DNA was from 1.0 ×10 -9 to 5.0 ×10 -6 mol/L,and the detection limit was 5.2 ×10 -10 mol/L.Conclusion The fabricated sensor is demonstrated to be sensitive and specific for the detection of Ebola virus nucleotide .
