Molecular epidemiological characteristics of metallo-β-lactamase-producing Pseudomonas aeruginosa from Changsha
10.3969/j.issn.1671-9638.2016.07.002
- VernacularTitle:长沙地区产金属β-内酰胺酶铜绿假单胞菌的分子流行病学特征
- Author:
Jingmin WU
;
Mingxiang ZOU
;
Jun LI
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
metallo-β-lactamase;
metalloenzyme;
genotype;
enterobacterial repetitive intergenic consensus-polymerase chain reaction
- From:
Chinese Journal of Infection Control
2016;15(7):447-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotypes and epidemic of metallo-β-lactamase-(MBL )-producing Pseudomonas aeruginosa (P .aeruginosa)in Changsha.Methods P .aeruginosa isolated from seven comprehensive hospitals in Changsha were collected and performed identification and antimicrobial susceptibility testing,pheno-types of MBL were detected with EDTA-disk synergy test and E-test,genotypes were determined by polymerase chain reaction (PCR),homology analysis were conducted by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR).Results Preliminary screening by EDTA-disk synergy test and E-test showed that only 10 of 81 iso-lates were strong positive;PCR result showed that 18 isolates were positive for MBL,11 of which were IMP-9-type MBL,1 was IMP-1-type,and 6 were VIM-2-type.SIM,SPM,GIM,and NDM-1-types were not found.ERIC-PCR showed that 12 strains of IMP-producing P .aeruginosa has multiple types,6 VIM-2-producing strains were of the same type.Conclusion IMP-9 and VIM-2 are main genotypes in P .aeruginosa in Changsha.
