Changes of NKT and MDSC during development of mouse lung tumor

VernacularTitle:小鼠肺肿瘤发展过程中外周血NKT和MDSC细胞变化
Author: Qianwen CAO ; Yu TIAN ; Tianjiao SONG ; Na WANG ; Xiaohui HAO ; Quanhui ZHENG
Publication Type:Journal Article
Keywords: Lung tumor; Nature killer T cells; Myeloid derived suppressor cells
From: Chinese Journal of Immunology 2016;32(9):1272-1275
CountryChina
Language:Chinese
Abstract: Objective:To investigate the change of nature killer T cell(NKT)and myeloid derived suppressor cells(MDSC) during the development of mouse lung tumor.Methods:Lung tumor mouse models were made by subcutaneous injection of Lewis lung tumor cells( LLC) ,peripheral blood leukocytes were extracted from mouse tail blood at different time points after LLC injection.NKT and MDSC were detected by flow cytometry after relative antibody staining.Results:With the increasing volume of lung tumor,the ratio of NKT cells decreased gradually,while the ratio of MDSC increased gradually in the peripheral blood of LLC-injected mice.Both NKT and MDSC showed significantly changes in LLC-injected mice compared with that of normal control mice.Conclusion:NKT and MDSC in LLC-injected mice show opposite changes during the development of lung tumor,so,they can be used as potential monitoring index for lung tumor development.