Study on PHBHHx co-cultured with mouse induced pluripotent stem cells for use as myocardial patches in vitro
10.3760/cma.j.issn.1001-4497.2016.07.009
- VernacularTitle:小鼠诱导多能干细胞与 PHBHHx 二维膜、三维膜体外共培养制作心肌补片
- Author:
Shijun XU
;
Junsheng MU
;
Jianqun ZHANG
;
Ping BO
- Publication Type:Journal Article
- Keywords:
Multipotent stem cells;
Ascorbic acid;
Myocytes,cardiac;
PHBHHx film
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2016;32(7):415-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of polymer material on adhesion , proliferation and differentiation of mouse in-duced pluripotent stem cells via co-culturing in vitro, to find a suitable polymeric biomaterials for miPSCs attachment , prolifera-tion and differentiation , forming myocardial patches as a new repair method for myocardial infarction .Methods miPSCs were recovered, passaged and cultured with PHBHHx two-dimension films and PHBHHx three-dimension films together.The mor-phology of miPSCs attached on the films was visualized under scanning electron microscope ( SEM) .The mouse induced pluri-potent stem cells were induced by differentiation medium that containing vitamin C .Control group did not add any inducer.The survival and differentiation of miPSCs were observed through immunofluorescence and flow cytometry .Results MiPSCs can grow, proliferate and differentiate on PHBHHx films both two-dimension and three-dimension.Vitamin C, as a favourable in-ducer, can markedly improve the efficiency of miPSCs differentiation of cardiomyocytes on PHBHHx films .Immunofluorescence results demonstrated positive cTnT expression.Flow cytometry measured cTnT expression: Vitamin C inducer group(47.54 ± 1.46)%>without any inducer group(7.02 ±0.95)%(P<0.05).In the process of miPSCs differentiating into myocardial cell, after adding Vitamin C, the expression quantity of cTnT:PHBHHx three-dimensional films(60.32 ±1.76)%>PHBHHx two-dimensional films(53.31 ±1.41)%>traditional cell culture(47.54 ±1.46)%(P<0.05).Conclusion iPSCs can ad-here, survive and differentiate on the PHBHHx film.Vitamin C, as a favourable inducer, can markedly improve the efficiency of miPSCs differentiation of cardiomyocytes .Relative to PHBHHx two-dimensional film culture and traditional culture , the three-dimensional PHBHHx film culture has a great advantage in the process of miPSCs differentiating into myocardial cells .
