Assay of Four Saponins in Astragali Radix by HPLC-ELSD
10.3870/j.issn.1004-0781.2016.08.015
- VernacularTitle:高效液相-蒸发光散射检测器测定黄芪中4种皂苷类成分
- Author:
Haixia CAI
;
Hongwei ZHAO
;
Yuhua QIN
;
Dongyan CHAI
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
Saponins;
Single marker;
Detector,high performance liquid chromatography-evaporative light scattering
- From:
Herald of Medicine
2016;35(8):861-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of high performance liquid chromatography-evaporative light scattering detector ( HPLC-ELSD) in quantitative analysis of multi-components ( QAMS) by single marker. Methods Four saponins in Astragali Radix were simultaneously determined by HPLC-ELSD using external standard method, and malonylastragaloside I served as internal standard. The relative correction factors between internal standard and astragaloside Ⅰ, astragaloside Ⅱ and astragaloside Ⅳ were calculated, and the stability was investigated. Results Astragaloside Ⅳ in Astragali Radix was little, while malonylastragalosideⅠand AstragalosideⅠwere abundant.The relative correction factors lacked stability, so ELSD could not be used in QAMS. Conclusion HPLC-ELSD can precisely determine contents of four saponins in Astragali Radix. The detector needs to be further studied when the components have poor ultraviolet absorption such as saponins by QAMS.
