Effects of chemokine MCP-1 on NMDA-mediated exciatory postsynaptic current in hippocampal slice of rats
10.3969/j.issn.1001-1978.2016.07.013
- VernacularTitle:趋化因子 MCP-1对大鼠海马区NMDA受体介导的兴奋性突触后电流的影响
- Author:
Shan LI
;
Zhe HU
;
Yan ZHOU
;
Huangui XIONG
- Publication Type:Journal Article
- Keywords:
chemokine;
MCP-1;
hippocampal slices;
EPSCs;
neuroexcitoxicity;
HAND
- From:
Chinese Pharmacological Bulletin
2016;32(7):950-955
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore how MCP-1 induces neu-rodisorder by determing the effects of MCP-1 on excita-tory postsynaptic current(EPSCs) in the CA1 region of rat hippocampal brain slices .Methods EPSCs, the AMPA receptor-mediated EPSC (EPSCAMPAR ), NMDA receptor mediated EPSCs(EPSCNMDAR) and NR2BR re-ceptor-mediated EPSC ( EPSCNR2BR ) were recorded u-sing whole-cell patch recording techniques to observe the effects of 2.3 nmol· L-1 MCP-1 on pyramidal neu-rons in hippocampal CA1 region.Microtubule-associat-ed protein-2 ( MAP-2 ) staining was used to study whether MCP-1 induced dendritic injuries in hippocam-pal CA1 region and whether NMDAR , AMPAR or CCR2 receptor antagonists had protective effects a-gainst dendritic damage caused by MCP-1.Results ① Bath application of MCP-1 produced a significant enhancement of the amplitudes of EPSCs , EPSCAMPAR and EPSCNMDAR .②Further studies revealed that MCP-1 potentiated EPSC NR2BR; ③ The MCP-1-associated dendritic injuries were blocked by NMDAR , AMPAR and CCR2R antagonists respectively .Conclusions Our results suggest a potential role of MCP-1 which may play in neuroexcitotoxicity and neural injury via NMDA receptor(especially NMDAR subtype NR2BR) and CCR2 receptor .The antagonists of these receptors may have potential therapeutic effect for neurodegener-ation.
