Detection of Hypoglycemic Drugs Illegally Added in Haokangshu Capsules by UPLC/MS/MS
- VernacularTitle:UPLC／MS／MS检测好康舒胶囊中非法添加降糖类化学药物
- Author:
Xuezhong GU
- Publication Type:Journal Article
- Keywords:
Haokangshu capsules;
UPLC-MS/MS;
Hypoglycemic agents;
Phenformin hydrochloride;
Glibenclamide;
Mass spectrometry
- From:
China Pharmacist
2016;19(7):1387-1391
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the hypoglycemic drugs illegally added in Haokangshu capsules .Methods: High performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used with an ACQUITY BEH C18 column(50 mm ×2.1 mm, 1.7 μm)and methanol-0.01 mol· L-1 ammonium acetate as the mobile phase with gradient elution .Through the molecular ion peak, the second order mass fragment ion information and MRM mode chromatographic peak retention time and the other information , the cap-sules containing metformin hydrochloride and 11 kinds of chemical hypoglycemic drugs were determined .Results:Through the analysis of 11 chemical hypoglycemic drugs , two chemical hypoglycemic agents , phenformin and glyburide , were detected out in the capsules . Conclusion:Phenformin and glibenclamide are illegally added in Haokangshu capsules .
