Simultaneous Determination of Three Flavonoids in Tianjihuang Granules by HPLC
- VernacularTitle:HPLC法同时测定田基黄颗粒中3种黄酮类成分的含量
- Author:
Dan ZHAO
;
Zulei DENG
;
Dezhou GE
;
Hao LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Tianjihuang granules;
Isoquercitrin;
Quercitrin;
Quercetin
- From:
China Pharmacist
2016;19(7):1415-1417
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the simultaneous determination of three flavonoids in Tianjihuang gran -ules.Methods:A ZORBAX Eclipse XDB-C18 column was used.The mobile phase was methanol-0.2%phosphoric acid solution (42∶58).The detection wavelength was 350 nm and the flow rate was 1.0 ml· min-1.Results:The linear range of isoquercitrin, quer-citrin and quercetin was 4.572-114.300 μg· ml-1(r=0.999 8), 7.028-175.700 μg· ml-1(r=0.999 8) and 2.195-54.880 μg· ml-1(r=0.999 9), respectively.The average recovery was 98.01%(RSD=1.24%, n=6), 98.14%(RSD=0.98%, n=6) and 97.00%(RSD=1.09%, n=6) , respectively.Conclusion:The method is specific and simple,which can be used for the quality con-trol of Tianjihuang granules.
