Role of endothelium in enhancement ofα1-adrenoceptor-mediated vasoconstriction by bupivacaine in isolated rat aorta
10.3969/j.issn.1001-1978.2016.07.015
- VernacularTitle:布比卡因增强α1受体介导大鼠胸主动脉收缩反应中血管内皮的作用
- Author:
Hefei ZHANG
;
Wenqi XU
;
Qian DU
;
Jing ZHAO
;
Hongyue XIA
;
Leiming REN
- Publication Type:Journal Article
- Keywords:
bupivacaine;
phenylephrine;
aorta;
vaso-constriction;
endothelium;
rat
- From:
Chinese Pharmacological Bulletin
2016;32(7):960-965
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of endothe-lium in the enhancement of phenylephrine-mediated vasoconstriction by bupivacaine in the isolated rat aor-ta.Methods The isolated rat aortic rings were pre-pared, and the vascular endothelium was removed chemically or physically .Phenylephrine-mediated vas-oconstriction was recorded .Results A pretreatment with bupivacaine at 30 μmol · L-1 for 20 min signifi-cantly increased the Emax value of vasoconstrictive re-sponses to phenylephrine from 2.22 ±0.07 g of sol-vent-controlled group to 2.50 ±0.05 g ( P<0.01 ) in the isolated endothelium-intact rat aorta.However, the Emax value was not significantly changed by a pretreat-ment with bupivacaine at 30 μmol · L-1 for 5 , 10 or 15 min ( P>0.05 ) .A pretreatment with bupivacaine at 30 μmol · L-1 for 20 min slightly but significantly inhibited the vasoconstrictive responses to low concen-tration of phenylephrine in the isolated endothelium-de-nuded rat aorta (P<0.05).In the isolated endotheli-um-intact rat aorta under a combined treatment with in-dometacin, ChTX, apamin and L-NAME, the vasodi-lator responses to acetylcholine were completely sup-pressed , and a pretreatment with bupivacaine at 30μmol· L-1 for 20 min did not significantly affect the vasoconstrictive responses to phenylephrine ( P >0.05 ) .Conclusion Bupivacaine enhances α1-adre-noceptor-mediated vasoconstriction by inhibiting vascu-lar endothelium in the isolated endothelium-intact rat aorta, Which potentiates indirectly the vasoconstrictive responses to phenylephrine .
