Effect of rosuvastatin on TLR4 signal pathway in rats with cardiac hypertrophy induced by pressure overload
10.3969/j.issn.1001-1978.2016.07.017
- VernacularTitle:瑞舒伐他汀对心肌肥厚大鼠 TLR4信号通路的影响
- Author:
Fangfang FAN
;
Lei ZHANG
;
Jiangjiu LIANG
- Publication Type:Journal Article
- Keywords:
rosuvastatin;
toll like receptor 4;
abdomi-nal aortic constriction;
pressure overload;
cardiac hy-pertrophy;
inflammation factors
- From:
Chinese Pharmacological Bulletin
2016;32(7):970-974
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the influence of rosuvastatin on toll-like receptor 4 ( TLR4 ) , and its downstream nu-clear transcription factor NF-kappa B p65, IκBα, in-flammation factors TNF alpha in rats with myocardial hypertrophy induced by pressure overload .Methods Myocardial hypertrophy was induced by abdominal aor-tic constriction ( AAC ) .Male Wistar rats were divided into 3 groups(n=10):① sham-operated rats(S);②AAC rats(M);③AAC+rosuvastatin(10 mg· kg -1· d-1 ) rats.From 1 week pre-opertion to 4 weeks post-operation, three groups were performed by gavage ad-ministration with equal volume of rosuvastatin and vehi-cle.The rats underwent cardiac color Doppler exami-nation.The level of ANP,TLR4, NF-κB p65,IκBα, TNF-αmRNA and protein expression in myocardium were detected by real-time PCR, Western blot, immu-nohistochemical and ELISA respectively .Results The sizes of heart and cardiomyocytes and the expression of ANP mRNA and TLR4 signaling molecules were signif-icantly increased in group M , which could be blocked by rosuvastatin .TLR4 protein was positively related to ANP, NF-κB p65 and TNF-αrespectively .Conclusion Rosuvastatin prevents cardiac hypertrophy induced by pressure overload , which is associated with its inhi-bition of TLR4, NF-κB and TNF-αin myocardium ex-pression .
