Measurement of serum heavy/light chain in monoclonal plasma cell proliferative disor-ders
10.3969/j.issn.1000-8179.2016.15.479
- VernacularTitle:血清重轻链检测在浆细胞病中的应用
- Author:
Congli ZHANG
;
Chen WANG
;
Jian LI
- Publication Type:Journal Article
- Keywords:
monoclonal protein;
serum heavy/light chain;
monoclonal plasma cell proliferative disorders
- From:
Chinese Journal of Clinical Oncology
2016;43(15):646-649
- CountryChina
- Language:Chinese
-
Abstract:
Monoclonal protein (M protein) is a serum surrogate used to conduct diagnostic, prognostic, and therapeutic evaluations of monoclonal plasma cell proliferative disorders. Two basic methods, namely, serum protein electrophoresis and immunofixation elec-trophoresis, are employed to detect and characterize M protein. Although these techniques have considerably improved, M protein quantification exhibits several drawbacks. In serum protein electrophoresis, M protein can migrate to various locations, and low M pro-tein levels cannot form a typical peak;as a consequence, additional problems in measurements arise. In 2009, a novel immunoassay in-volving a heavy/light chain (HLC) was developed. HLC recognizes immunoglobulins with specific heavy and light chain isotypes. The ra-tio between an involved monoclonal immunoglobulin and an uninvolved background polyclonal immunoglobulin can be calculated through immunoglobulin quantitation by using isotype-specific light chains. This review summarizes relevant parameters that provide diagnostic, prognostic, and therapeutic data regarding monoclonal plasma cell proliferative disorders.
