Effects of immunostimulator CH2 b on mixed peptides derived from glucose-6-phosphate isomerase induces arthritis
10.3969/j.issn.1000-484X.2016.08.002
- VernacularTitle:免疫调节剂CH2b对葡萄糖－6－磷酸异构酶混合肽段诱导的类风湿性关节炎小鼠的影响
- Author:
Jialin LIU
;
Xuejiao ZHANG
;
Fei YANG
;
Yongfu LOU
;
Ming MENG
;
Dongzhi CHEN
- Publication Type:Journal Article
- Keywords:
Immunostimulator CH2b;
α-GalCer;
Glucose-6-phosphate isomerase;
iNKT cells
- From:
Chinese Journal of Immunology
2016;32(8):1094-1098
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of the synthetic immunomodulator CH 2b with a thiazolidin-4-one ring on the pathogenesis of rheumatoid arthritis (RA) mice induced by glucose-6-phosphate isomerase(GPI) mixed peptides.Methods:hGPI325-339 ,hGPI469-483 peptide fragments were mixed with complete freund′s adjuvant fully ,DBA/1 mice were givien subcutaneous injection of the emulsifiers,pertussis toxin to strengthen immunity.And then RA mice were intervened with α-GalCer and CH2b,the changes of body weight ,ankle joint symptoms scores were observed .The joint tissues stained with hematoxylin and eosin ( HE) was used to evaluate the inflammatory cells.Fluorescence-activated cell sorting ( FACS) was used to detect the frequency changes of iNKT cells .Cytometric bead array(CBA) was used to analyze the levels of serum cytokines TNF-α,IL-6,IL-4,IFN-γ.Results: Compared with the model group,α-GalCer,CH2b could reduce the inflammation of the model mice ,significantly improve the body weight growth and the joint swelling(P<0.05).The inflammatory cells infiltration were decreased.More importantly,CH2b improved the frequency of iNKT cells in peak,the difference was statistically significant (P<0.05).The levels of TNF-α,IL-6,IFN-γin serum were significantly decreased(P<0.05) by CH2b.α-GalCer,CH2b could decrease the levels of TNF-α,IL-6,IFN-γ,and the two groups had no statistically significant difference(P>0.05).Conclusion:Immunomodulator CH2b by activating iNKT cells affect the secretion of inflammatory cytokines ,and it relieved the GPI induced arthritis .
