Effect of DDC on activity of SOD of Bifidobacteria infantis
10.3969/j.issn.1000-484X.2016.08.004
- VernacularTitle:DDC对婴儿双歧杆菌SOD酶活性的影响
- Author:
Yan LUO
;
Lan GE
;
Hongbing CHEN
;
Pingchang YANG
;
Zhigang LIU
;
Bo YANG
- Publication Type:Journal Article
- Keywords:
Diethyl dithiocarbamate;
Inhibitor;
Bifidobacterium infantis;
Superoxide dismutase
- From:
Chinese Journal of Immunology
2016;32(8):1103-1106
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether diethyldithiocarbamate (DDC) affect the activity of superoxide dismutase (SOD) of Bifidobacterium infantis ( BB ) .Methods: We used simple anaerobic activated ways to culture BB .After the bacterium reached platform growth period ,two concentrations (200 and 1 000 μmol/L) of DDC were added to bacterium medium for a specific period of time (0.5,1,2,4 and 24 h),which was in order to determine if this inhibitor affected the activity of SOD .As a positive control,the in-hibitory effect of DDC on the activity of SOD in murine intestinal samples was also checked .Results:It was found that incubation of BB with DDC for some specific periods showed no effect on the activity of SOD .However , the activity of SOD in intestine reduced dramatically after the mice were given DDC in vivo .Conclusion:DDC affect the SOD activity in mice ,while it showed no effect on SOD activity of BB.The suitable inhibitor for SOD of BB is not commercially available and further research is warranted .
