Expression of STAT3 and IL-6 in epilepsy rat caused by penicillin

VernacularTitle:青霉素诱导的大鼠癫痫模型中STAT3及IL－6表达
Author: En CHEN ; Lin LIN ; Saiping Lü ; Jieni CHEN ; Chunjing CHEN ; Xuesen ZOU
Publication Type:Journal Article
Keywords: Epilepsy; Rat; STAT3; IL-6
From: Chinese Journal of Immunology 2016;32(8):1112-1117
CountryChina
Language:Chinese
Abstract: Objective:To investigate the expression of IL-6 and STAT3 in the epilepsy rat caused by penicillin .Methods:The model of epilepsy in rats was made by abdominal injecting penicillin .Then the EEG and behavior was observed in each group and the level of IL-6/STAT3 transcription and cytokine IL-6 secretion in different groups was compared using qRT-PCR and ELISA respectively.The protein expression of STAT3 was detected using Western blot.Results: 500 U/kg and 800 U/kg rats injected penicillin were induced epilepsy and show typical epileptic seizures behavioral and brain waves ,seizures was 100%;the level of IL-6/STAT3 transcription and IL-6 secretion in the model of epilepsy was significantly higher than control .There was no significant difference in the expression of STAT3 protein levels,but a significant increase in p-STAT3.Conclusion:Expression of STAT3 and IL-6 plays an important role in the Epilepsy Rat caused by penicillin .