Effect of 3-MA on camptothecin-induced cervical cancer Hela cell apoptosis
10.3969/j.issn.1000-484X.2016.08.009
- VernacularTitle:3－甲基腺嘌呤对喜树碱诱导的宫颈癌Hela细胞凋亡的影响
- Author:
Xiaona WANG
;
Laifeng REN
;
Anjiang ZHAO
;
Wanxia YANG
;
Yunqing REN
- Publication Type:Journal Article
- Keywords:
3-methyladenine;
Camptothecin;
Autophagy;
Apoptosis;
Helacells
- From:
Chinese Journal of Immunology
2016;32(8):1128-1132
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of autophagy inhibitor 3-methyladenine(3-MA) on camptothecin(CPT)-induced Hela cell apoptosis.Methods:MTT assays were carried out to determine the optimal concentration and time of CPT on Hela cells and the effect of different drugs on Hela cell proliferation activity .After Hela cells were treated with different drugs ,the changes of autophagy marker protein( microtubule-associated protein 1 light chain 3,LC3),p62 and apoptosis-related protein were detected using Western blot and immunofluorescence ( IF) .DAPI ( nuclear ) staining was used to observe cell apoptosis rate .Results: In CPC-treated Hela cells,Hela cell proliferation activity declined dramatically ,and autophagy could be induced to occur .Compared with CPT group ,the cell proliferation activity was lower in CPT combined with 3-MA group,the level of autophagy decreased ,but the apoptosis rate significantly increased.Conclusion:CPT can induce autophagy while inducing Hela cell death .Hela cells chemosensitivity to CPT treatment can be enhanced by 3-MA inhibiting autophagy .
