Expression of miR-634 in hepatocellular carcinoma and its effect on biological behavior of Hepatocellular carcinoma cells
10.3969/j.issn.1000-484X.2016.08.017
- VernacularTitle:miR－634在肝癌中的表达及对肝癌细胞生物学行为的影响
- Author:
Fei LI
;
Dexin LI
;
Guangpeng ZHOU
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
miR-634;
Proliferation;
Invasion;
Apoptosis
- From:
Chinese Journal of Immunology
2016;32(8):1160-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression level of microRNA-634 (miR-634) in hepatocellular carcinoma (HCC) and its regulatory effect on the common biological behavior of hepatocellular carcinoma cells .Methods: Real-time fluorescence quantitative PCR (RT qPCR) method was used to detect HCC cell lines (HepG2, SMMC7721, BEL7402, bel7404, SNU739), 69 cases of HCC tissues and matching relative quantification of miR-634 paracancerous tissues and analysis of relationship between miR-634 expression and HCC patients gender, age, tumor size, degree of differentiation, child Pugh classification, BCLC staging, portal vein tumor thrombus and liver metastasis , while building a miR-634 eukaryotic expression vector and transfected into hepatocellular carcinoma cell lines, using live cell counting kit-8 CCK-8, flow cytometric annexin V/PI double staining and Transwell experiment to detect the transfection miR-634 on cell proliferation , apoptosis and invasion effects .Results:Compared with the normal human liver cell line L-02 and hepatocellular carcinoma cells miR-634 were decreased ( P<0.05 ) , the expression followed by HepG 2>SNU739>Bel7402>Bel7404>SMMC7721;69 cases of hepatocellular carcinoma ( HCC ) of miR-634 level ( 0.253 ±0.019 ) and lower than that of the matched paracancerous tissues ( P<0.05 ) , and related with the tumor size , degree of differentiation , BCLC stage , portal vein tumor thrombus and liver metastasis ( P<0.05 ) .Over expression in the transfected group 24-96 h after miR-634 level continues to rise , control group and blank vector transfected group differences were statistically significant ( P<0.05 );and the control transfection group and blank group compared to transfection proliferation inhibition rate , apoptosis rate was increased , but wear the number of cell membrane decreased, the difference was statistically significant (P<0.05).Conclusion:miR-634 in hepatocellular carcinoma tissues and cells were low expression and related to clinical pathological parameters , raised its level can inhibit the proliferation and invasion of hepatocellular carcinoma cells and induce apoptosis , for the prevention and treatment of liver cancer has an important reference value .
