Comparison of chemiluminescent microparticle immunoassay and enzyme-linked immunosorbent assay for determination of anti-neutrophil cytoplasm antibodies
10.3969/j.issn.1000-484X.2016.08.020
- VernacularTitle:磁微粒化学发光法与酶联免疫吸附法测定抗中性粒细胞胞浆抗体的结果比较
- Author:
Lijuan ZHANG
- Publication Type:Journal Article
- Keywords:
Anti-neutrophil cytoplasm antibodies (ANCA);
Myeloperoxidase(MPO);
proteinase 3(PR3);
Enzyme-Linked Im-munoSorbent Assay;
Chemiluminescent Microparticle ImmunoAssay
- From:
Chinese Journal of Immunology
2016;32(8):1175-1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the performance of chemiluminescent microparticle immunoassay ( CMIA) and enzyme-linked immunosorbent assay ( ELISA) for the determination of Anti-PR3 and Anti-MPO.Methods:Concentration of Anti-PR3 and Anti-MPO in serum samples from 166 patients with autoimmune diseases and 50 healthy donors were determined by using CMIA (Method A) and ELISA(Method B),respectively.The results from both assays were analyzed and compared by statistical methods .Results:Method A showed better intra-assay reproducibility and inter-assay reproducibility than Method B for the determination of high ,medium and low levels of control serum .Both methods met the accuracy requirement .The correlation coefficient of Anti-PR3 and Anti-MPO were 0.987 8 and 0.989 6 for Method A and Method B ,respectively.And the Kappa coefficients were 0.897 and 0.882 for Method A and Method B,respectively.Conclusion:The performance of Method A is superior to Method B for the deter-mination of Anti-PR3 and Anti-MPO, which makes Method A to be a potentially better choice for clinical application .
