Effects of naringin on growth and COX-2 expressions in human cervical cancer HeLa cell line

VernacularTitle:不同浓度柚皮苷对人宫颈癌HeLa细胞体外增殖及其COX－2表达影响
Author: Wenjing YANG ; Lu WANG ; Rui SUN
Publication Type:Journal Article
Keywords: Naringin; HeLa cell; Growth; COX-2
From: Chinese Journal of Immunology 2016;32(8):1200-1203
CountryChina
Language:Chinese
Abstract: Objective: To study the effect of naringin on human cervical cancer HeLa cell line and its related mechanism.Methods:HeLa cells were cultured in vitro in the presence of different concentration naringin .The method of MTT was used to detect the growth of HeLa cell in 24 h, 48 , 72 h.Apoptotic cells were observed by Hoechst 33258 staining.The protein expressions of COX-2 in SCL-12 cells were analyzed by Western blot .Results: The inhibition ratio of the cell growth in middle dose group and high dose group significantly higher than control group ( P<0.05 ) and the inhibition ratio in low dose group was no significant change(P>0.05).The apoptosis ratio of the cell growth in middle dose group and high dose group significantly higher than control group( P<0.05 ) and the apoptosis ratio in low dose group was no significant change ( P>0.05 ) .The expression of COX-2 was significantly decreased in middle dose group and high dose group .Conclusion: Naringin plays a central role in the apoptosis of the human cervical cancer HeLa cell line ,which may be related to the expression of COX-2.