The effect of ropivacaine on proliferation and cell cycle of human breast cancer MCF-7 cells
- VernacularTitle:不同浓度罗哌卡因对乳腺癌 MCF-7细胞增殖和细胞周期的作用研究
- Author:
Ming XIA
;
Shaohua WANG
;
Jianhua TONG
;
Yuanhui TAN
;
Zhiqiang ZHOU
;
Jianguo XU
- Publication Type:Journal Article
- Keywords:
Ropivacaine;
Breast cancer;
Proliferation;
Cell cycle
- From:
The Journal of Clinical Anesthesiology
2016;32(7):680-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the different behavior of proliferation and cell cycle of MCF-7 cells when exposured to ropivacaine of different concentrations and further explore its underlying mechanism.Methods Human breast cancer cells MCF-7 were inoculated into culture medium for 24 h,then were randomly divided into four groups:Control group(group C),Ropivacaine 100 μg/ml group(group R1 ),Ropivacaine 200 μg/ml group(group R2),Ropivacaine 400 μg/ml group(group R3).We medicated each group and incubated for 48 h,then detected the cell proliferation and cell cy-cle immediately.The level of protein TCF-4 and beta-catein of groups R3 and C were measured at the same time.Results MCF-7 cell viability of groups R2 and R3 was significantly lowed (P <0.05 ), MCF-7 cell viability of group R1 had no significant difference when compared to group C.G0/G1 phase cells of groups R1,R2 and R3 were significantly less than those of group C,S phase cells of groups R1,R2 and R3 were significantly more than group C,G2/M phase cells of groups R1,R2 and R3 were significantly more than group C (P <0.05).The expression level of TCF-4 and beta-catenin in group R3 was significantly lower than that in group C (P <0.05).Conclusion Ropivacaine inhibits the proliferation of breast cancer cells MCF-7 by down-regulating TCF-4 and beta-cateni.
