The effect of pre-treatment with an Nrf2 inducer dh404 on renal ischemia reperfusion injury in rats
- VernacularTitle:Nrf2诱导剂 dh404预处理对大鼠肾脏缺血-再灌注损伤的保护作用
- Author:
Meiling DENG
;
Yongqiao HUANG
;
Yunxia GU
;
Yingping LIANG
;
Yanhui HU
;
Guohai XU
- Publication Type:Journal Article
- Keywords:
CDDO-9,1 1-dihydro-trifluoroethyl amide;
Nuclear factor erythroid-derived factor 2-related factor;
Kidney;
Ischemia reperfusion injury;
Anti-oxidant
- From:
The Journal of Clinical Anesthesiology
2016;32(7):700-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of pre-treatment with an Nrf2 inducer dh404 on renal ischemia reperfusion injury in rats.Methods Thirty SD rats were divided into three groups using completely randomized digital table,dh404 was dissolved in sesame oil and was given orally 1.5 mg/kg the night before procedures and 5 hours before procedures.Rats in group Sham received no treatment of ischemic reperfusion.In group IR and group dh404,the renal ischemia reper-fusion (IR)model was established,24 hours after IR,the levels of serum creatininc (Cr)and urea ni-trogen (BUN),the activities of superoxide dismutase (SOD)and the content of malonaldehyde (MDA)in serum were measured,and hematoxylin eosin(HE)staining observe the changes in renal structure,the levels of γ-glutamate-cysteine ligase catalytic (GCLC)and modifier (GCLM)subunit, the expression of NF-κB,COX-2 and eNOS were measured.Results Compared with group Sham,the values of Cr,BUN in group IR and group dh404 were significantly higher (P <0.05).Compared to the group IR,the group dh404 Cr,BUN values significantly decreased after reperfusion for 24 h(P <0.05 ).Compared to group Sham,group IR SOD activity decreased,while the value of MDA increased(P <0.05 ).Compared to group IR,group dh404 had much higher SOD activity,while the value of MDA significantly decreased.Observed with optical microscopy,compared to group Sham, the renal tubular injury of group IR was obvious.Compared to group IR,group dh404 significantly reduced tubular injury.Compared to group IR,the levels of GCLC and modifier GCLM subunit were higher,while there were no significant differences of levels among NF-κB,COX-2 and eNOS. Conclusion Pre-treatment with an Nrf2 inducer dh404 can protect the kidney from IRI through possi-bly reducing IRI kidney oxidative stress.
