Influence of vitamin D on proliferation and differentiation of human preadipocytes in high-glucose con-dition
10.3760/cma.j.issn.1674-635X.2016.03.006
- VernacularTitle:高糖环境下维生素D对人前脂肪细胞增殖分化的影响
- Author:
Hongxia ZHANG
;
Jie YUAN
- Publication Type:Journal Article
- Keywords:
Vitamin D;
Vitamin D receptor;
Obesity;
Diabetes mellitus
- From:
Chinese Journal of Clinical Nutrition
2016;24(3):155-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of 1,25(OH)2D3 (VD3) on proliferation and dif-ferentiation of human preadipocytes in high-glucose condition .Methods Primary culture of human preadipo-cytes were divided into 4 groups:the control group, high glucose group, control+VD3 group, and high glucose+VD3 group.Oil red O staining and extraction with isopropyl alcohol were employed to detect the total amount of fat in different groups .Real-time polymerase chain reaction was used to detect the mRNA levels of vitamin D receptor (VDR), CCAAT/enhancer binding protein a (C/EBPa) and peroxisome proliferator activated receptor gamma ( PPARγ) in each group .Results Compared with the control group , the fat amount was significantly increased in the high glucose group (0.231 ±0.033 vs.0.362 ±0.045 , P=0.037 ) and high glucose +VD3 group (0.231 ±0.033 vs.0.358 ±0.057, P=0.033), decreased in the control +VD3 group (0.231 ± 0.033 vs.0.174 ±0.029, P=0.016), but no significant difference in the glucose+VD3 group (0.362 ±0.045 vs.0.358 ±0.057, P=0.450);VDR mRNA level was decreased in the high glucose group (0.397 ± 0.029 vs.0.069 ±0.003 , P=0.022 ) , but VD3 increased the VDR mRNA levels in both the control +VD3 group and the high glucose +VD3 group (0.397 ±0.029 vs.1.103 ±0.044 , P=0.035;0.069 ±0.003 vs. 0.152 ±0.021 , P=0.041 );the mRNA levels of C/EBPa and PPARγwere up-regulated in the high glucose group (0.135 ±0.048 vs.0.788 ±0.063, P=0.018;0.498 ±0.026 vs.0.795 ±0.037, P=0.021), VD3 decreased the PPARγmRNA level in the control +VD3 group ( 0.498 ±0.026 vs.0.237 ±0.011 , P =0.029), but had no significant effect on C/EBPa mRNA (0.788 ±0.063 vs.0.843 ±0.049, P>0.05) and PPARγmRNA (0.795 ±0.037 vs.0.813 ±0.041, P>0.05) expressions in the high glucose +VD3 group or C/EBPa mRNA in the control+VD3 group (0.135 ±0.048 vs.0.156 ±0.022, P>0.05).Conclusion VD3 may lose the ability to inhibit the proliferation and differentiation of human preadipocyte due to reduced biologi -cal activity when exposed to high-glucose environment in vitro.
