Determination of Norcantharidin in Norcantharidin in situ Gel by HPLC
- VernacularTitle:高效液相色谱法测定去甲斑蝥素原位凝胶中去甲斑蝥素的含量
- Author:
Yujie HU
;
Feng ZHOU
;
Xinjun CAI
- Publication Type:Journal Article
- Keywords:
Norcantharidin;
In situ gel;
HPLC
- From:
China Pharmacist
2016;19(6):1196-1197
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of norcantharidin in norcantharidin in situ gel .Methods:An optimal HPLC method was set up and an Agilent ZORBAX SB-C18 column (150 mm ×4.6 mm, 5μm) was adopted.The mobile phase was acetonitrile-phosphate buffer solution(1∶9, adjusting pH to 3.1 with phosphorjc acid).The flow rate was 0.8 ml· min-1 and the column temperature was 25℃.The detection wavelength was set at 210 nm and the injection volume was 20μl.Results:Norcanthari-din had a good linear relationship within the range of 0.02-1.00 mg· ml-1 (r=0.999 9).The average recovery was 97.5%and RSD was 0.98%(n=9).Conclusion:The method is accurate, simple and reproducible, which can be used for the determination of nor-cantharidin in norcantharidin in situ gel .
