Determination of Vitamin B6 and Fluocinonide in Compound Vitamin B6 Gel by HPLC
- VernacularTitle:HPLC法测定复方维生素 B6凝胶剂中维生素 B6与醋酸氟轻松的含量
- Author:
Wanli JI
;
Hui YAO
;
Dingwen SHEN
- Publication Type:Journal Article
- Keywords:
Vitamin B6;
Fluocinonide;
HPLC;
Gels
- From:
China Pharmacist
2016;19(6):1214-1216
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine vitamin B 6 and fluocinonide in compound vitamin B 6 gel.Methods:The contents of vitamin B6 and fluocinonide in the gel were determined by HPLC with a Lichrospher CN C 18column (250 mm ×4.6 mm, 5μm).The mobile phase was heptane sulfonate solution (adding 6.8 g potassium phosphate monobasic and 1.0 g heptane sulfonate into 1000 ml water) -methanol –triethylamine (35:65:0.2,adjusting pH to 3.2 with phosphoric acid).The detection wavelength was 238 nm and the column temperature was 25℃.The injection volume was 20 μl.Results: Vitamin B6 had a good linear relationship within the range of 15.0-300.0 μg· ml-1(r=1.000 0), and the average recovery was 99.65% (RSD=0.3, n=9).Fluocinonide had a good linear relationship within the range of 0.4-8.0 μg· ml-1 (r=0.9990), and the average recovery was 99.35% (RSD=0.85, n=9).Conclusion:The method is simple and reproducible, and the result is accurate.The method can be used for the deter-mination of the gel.
