Application of Real-time Fluorescent Quantitative PCR in the Identification of Fritillariae Cirrhosae Bulbus
- VernacularTitle:实时荧光定量 PCR对川贝母的鉴别应用
- Author:
Dalong LUO
;
Linjie HUANG
;
Lin HUANG
- Publication Type:Journal Article
- Keywords:
Real-time fluorescence quantitative PCR;
Magnetic beads;
Fritillariae Cirrhosae Bulbus;
Identification
- From:
China Pharmacist
2016;19(6):1068-1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of real-time fluorescence quantitative PCR in the identification of Fritillariae Cirrhosae Bulbus.Methods:The DNA of samples was extracted by magnetic beads ,the primers were amplified by real-time fluores-cence quantitative PCR , and the Cq value and amplification curve were used to determine the samples .Results:The Cq values for five batches of Fritillariae Cirrhosae Bulbus were lower than 30, and the curve had obvious growth period .No Cq value was shown for Fritil-lariae Ussuriensis Bulbus , Fritillariae Thunbergii Bulbus and Bolbostemmatis Rhizoma with straight line curves .Conclusion:The meth-od is simple,feasible and effective in the identification of Bulbus Fritillariae Cirrhosae with high accuracy and good reproducibility .
