Analysis of Fetal Nucleated Erythrocytes in Maternal Blood by Fluorescence In Situ Hybridization(FISH).
- Author:
Kowan Ja JEE
1
;
Seok Chan KIM
;
Goo Ryun KIM
;
Ill Woon JI
;
Jae Hyun CHUNG
;
Young Kyu MOON
Author Information
1. Moons Obstetrics and Gynecology Clinic, Genetic Institute, Korea.
- Publication Type:Original Article
- Keywords:
Fetal cells(nRBC);
FISH;
Sensitivity and specificity;
Aneuploidy
- MeSH:
Aneuploidy;
Diagnosis;
Erythroblasts*;
Female;
Fetus;
Fluorescence*;
Humans;
Pregnant Women;
Sensitivity and Specificity
- From:Korean Journal of Obstetrics and Gynecology
1997;40(5):1019-1023
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The isolation of fetal cells from maternal circulation has the potential to allow relativelyself prenatal diagosis for all pregnant women. The present technology, however, has notreached the accuracy required for clinical diagnosis because of maternal cell contaminationSo we published a new method for enrichment of nRBC in a fetal cell isolation(1996).In this study, attempted to FISH analysis of nRBC which was isolated by our ownmethods. We evaluated the efficiency of FISH.As the results, we have successfully used FISH on enriched nRBC.We were able to identified 2 abnormal fetus which were confirmed by conventionalcytogenentic study as Down syndrome(Fig.1) and Klinefeltre syndrome(Fig.2). And thesensitivity and specificity for FISH was 86%(49/57) and 92.3%(36/39), respectively.According to our results, fetal cell analysis by FISH can be reliable used for prenatalaneuploidy diagnosis. However, the problems of enrichment of the fetal cell and FISH probeor condition should be over come before analyze.
