Simultaneous Determination of Four Glycosides in Microctis Folium by UPLC
- VernacularTitle:UPLC 同时测定布渣叶中四个苷类成分的含量
- Author:
Wenhui LUO
;
Zhao CHEN
;
Aili XU
;
Jieyi JIANG
;
Weitao CHEN
- Publication Type:Journal Article
- Keywords:
Microctis folium;
Narcissoside;
Isovitexin;
Kaempferol-3-rutinoside;
Isorhamnetin-3-O-glucoside;
UPLC
- From:
China Pharmacist
2016;19(6):1079-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a UPLC method for determining four glycosides in microctis folium.Methods:The UPLC deter-mination was performed on an Agilent SB -C18 (3.0 mm ×50 mm, 1.8 μm) colume with 0.05% acetonitrile -phosphate solution as the mobile phase.The detection wavelength was set at 320 nm.The flow rate was 0.3 ml· min-1.The column temperature was 25℃.Results:Narcissoside, isovitexin, kaempferol-3-rutinoside and isorhamnetin-3-O-glucoside all showed good linearity with the re-covery of 98.89%,99.03%, 97.00%and 97.41%, and RSD of 0.41%, 0.84%,0.44%and 0.80%, respectively (n=9).Con-clusion:The method is convenient with good stability , repeatability and sample recovery rate , which provides basis for the quality eval-uation and control of microctis folium.
