Determination of Dracorhodin and Curcumin in Dieda Pills by UPLC
- VernacularTitle:UPLC 测定跌打丸中血竭素与姜黄素的含量
- Author:
Chunhui ZHANG
;
Yu YU
;
Guoping WANG
;
Chao FU
;
Youlan WANG
- Publication Type:Journal Article
- Keywords:
Dieda pills;
Dracorhodin;
Curcumin;
UPLC;
Content determination
- From:
China Pharmacist
2016;19(6):1206-1208
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the content of dracorhodin and curcumin in Dieda pills by UPLC .Methods:A UPLC method was adopted.The determination was performed on a Waters Acquity UPLC BEH C 18 column(100 mm ×2.1 mm,1.7 μm) with the mobile phase consisting of acetonitrile-0.05 mol· L-1 NaH2PO4(50 ∶50).The detection wavelength was 440 nm for dracorhodin and 431 nm for curcumin, the column temperature was 30℃and the flow rate was 0.1 ml· min-1 .Results:There was a good linear range of 0.001 8-0.036 4 μg(r=0.999 9)for dracorhodin and 0.000 8-0.015 6 μg(r=0.999 9) for curcumin.The average recovery for dracorhodin was 97.94%(RSD=0.89%) and that for curcumin was 98.45%(RSD=0.91%).Conclusion:The method is simple, rapid and reproducible ,which can be used for the determination of dracorhodin and curcumin in Dieda pills .
