Determination of Schaftoside in Jinshaniu Huashi Tablets by HPLC
- VernacularTitle:HPLC法测定金沙牛化石片中夏佛塔苷含量
- Author:
Zai LIU
;
Li ZHANG
;
Huizhen KUANG
;
Mingguo DONG
- Publication Type:Journal Article
- Keywords:
Jinshaniu Huashi tablets;
Schaftoside;
HPLC;
Content determination
- From:
China Pharmacist
2016;19(6):1224-1225
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method to determine the content of schaftoside in Jinshaniu Huashi tablets .Meth-ods:The separation was performed on an Agilent ZORBAX SB-C18 column(150 mm ×4.6 mm, 5 μm)with 0.2% phosphoric acid-acetonitrile as the mobile phase with gradient elution at a flow rate of 1.0 ml· min-1 .The detection wavelength was set at 272 nm, and the column temperature was set at 25 ℃.Results: The calibration curve of schaftoside had a good linearity within the range of 4.58-183.15 μg· ml-1(r=1.000 0).The average recovery was 99.65%with RSD of 1.09%(n=6).Conclusion:The established method is simple and accurate with high repeatability , which can be used for the content determination of schaftoside in Jinshaniu Hua-shi tablets .
