Simultaneous Determination and Recognition Analysis of Coumarins in Angelica decursiva and Peucedanum praeruptorum by HPLC-DAD.
10.20307/nps.2016.22.3.162
- Author:
Hye Mi KIM
1
;
Su Yang JEONG
;
Sun Min KIM
;
Kyu Ha LEE
;
Jong Hwan KIM
;
Rack Seon SEONG
Author Information
1. Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Chungcheongbuk-do 28159, Korea. seongrs@korea.kr
- Publication Type:Original Article
- Keywords:
Peucedani Radix;
Angelica decursiva;
Peucedanum praeruptorum;
coumarin;
HPLC-DAD
- MeSH:
Angelica*;
Asian Continental Ancestry Group;
Chromatography, High Pressure Liquid;
Coumarins*;
Humans;
Limit of Detection;
Methods;
Plants, Medicinal;
Quality Control;
Water
- From:Natural Product Sciences
2016;22(3):162-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
Peucedani Radix is the root of Angelica decursiva Franchet et Savatier (=Peucedanum decursivum Maximowicz) or Peucedanum praeruptorum Dunn in several Asian countries. The coumarins contained in Peucedani Radix were quantitatively analyzed using HPLC-DAD to develop a simultaneous determination for the quality control of A. decursiva and P. praeruptorum. For quantitative analysis, four major coumarins contained in these medicinal plants were assessed. Nodakenin (1), nodakenetin (2), praeruptorin A (3), and praeruptorin B (4) were separated with a Phenomenex Luna C18 column (5 µm, 4.6 × 250 mm) under the gradient conditions using distilled water with 0.1% phosphoric acid and acetonitrile with 0.1% phosphoric acid as the mobile phase, at a flow rate of 1.0 ml/min and a detection wavelength of 330 nm. This method was fully validated for linearity, accuracy, precision, recovery, and limit of detection and quantification. As a result, A. decursiva and P. praeruptorum were clearly classified by the quantification of four major coumarins in extracts. Also, the pattern recognition analysis based on HPLC indicates that all of the samples were largely clustered into two groups. Therefore, it is possible to distinguish between A. decursiva and P. praeruptorum and contribute to quality control.
