QDPR gene expression level influences oxidative stress of NRK-52E cells in high glucose
10.3760/cma.j.issn.1000-6699.2016.06.013
- VernacularTitle:QDPR基因表达变化对高糖环境下NRK-52 E细胞氧化应激的影响
- Author:
Lingyu MENG
;
Xuejing WU
;
Zhijie PU
;
Xiangjun YANG
;
Xiaolu ZHANG
;
Shasha ZHAO
;
Jingyi ZHANG
;
Haojun ZHANG
;
Zhiguo LI
- Publication Type:Journal Article
- Keywords:
Quinoid dihydropteridine reductase;
Diabetic nephropathy;
Oxidative stress;
Renal tubular epithelial cells
- From:
Chinese Journal of Endocrinology and Metabolism
2016;32(6):499-504
- CountryChina
- Language:Chinese
-
Abstract:
_ Objective_ To study whether quinoid dihydropteridine reductase ( QDPR ) expression level change can affect oxidative stress of NRK-52E renal tubular cells in a high glucose environment. Methods The NRK-52E model of overexpression, knockdown QDPR gene and respective control were constructed by lentivirus. All groups were given 5. 4 mmol/L and 30 mmol/L glucose culture medium respectively to imitate normal and high glucose condition. The level of superoxide anion ( O-2 ) was detected by flow cytometer dihydroethidium method. The protein expression level of superoxide dismutase 1 (SOD1)was tested by Western blot. Results QDPR over-expression can decrease O-2(P<0. 01)and SOD1(P<0. 05)levels in high glucose condition;QDPR knockdown increases O-2(P<0. 01) and does not change SOD1. Conclusion Under high glucose condition, overexpression of QDPR gene decreases NRK-52E cell oxidative stress. Knockdown QDPR gene increases NRK-52E cell oxidative stress. QDPR gene may influence the development of diabetic nephropathy by oxidative stress.
