Advances in carbapenemase detection in Gram-negtive bacilli
10.3760/cma.j.issn.1674-2397.2016.03.008
- VernacularTitle:革兰阴性杆菌碳青霉烯酶检测方法的研究进展
- Author:
Chenrui HOU
;
Yun YANG
;
Ziyang LI
;
Ruixue WANG
;
Chunyan GAO
;
Lili DING
;
Miao SU
;
Chaojun LANG
;
Jianrong RONG
- Publication Type:Journal Article
- Keywords:
Gram-negative bacteria;
Carbapenemase;
Drug resistance;
Phenotype;
Genotype
- From:Chinese Journal of Clinical Infectious Diseases
2016;9(3):248-254
- CountryChina
- Language:Chinese
-
Abstract:
With increasing use of carbapenem antibiotics , carbapenems-resistant gram-negative bacteria are spreading, and carbapenemase-producing is the main mechanism of carbapenems resistance . Rapid and accurate identification of carbapenemase and its type is of great importance to timely and effective treatment and control of infections .Chromogenic /Fluorogenic culture media, modified Hodge test and double disk synergy test are traditional methods for carbapenemase detection , but all are time-consuming. Biochemical method is more time efficient and with high sensitivity and specificity , but cannot be used to identify subtypes.Now matrix-assisted laser desorption ionization -time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully applied in the identification of species , subtypes and detection of drug -resistant genes.And among various carbapenemase gene detection techniques , next generation sequencing (NGS) can also be used for the detection of integrons , transposons and plasmids, which is important in both epidemiology and resistant mechanism studies .This article reviews the advantages and disadvantages of various methods for phenotype and gene detection of carbapenemase .
