Construction and expression of eukaryotic expression vector of human IL-37 b gene
10.3969/j.issn.1005-4847.2016.03.010
- VernacularTitle:人IL-37b基因真核表达载体的构建与表达
- Author:
Jing YAO
;
Jiang CHENG
;
Xuefeng PEI
;
Jingyu WANG
;
Ming YUAN
- Publication Type:Journal Article
- Keywords:
Interlukin-37;
Gene cloning;
Eukaryotic expression vector;
THP-1 cells
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression vector pEGFP-N1/IL-37b and analyze the expression of IL-37 gene in THP-1 cells. Methods Total RNA was extracted from human peripheral blood mononuclear cells ( PB-MCs) and the coding region of IL-37b gene was amplified by RT-qPCR. Then, the gene was cloned into pEGFP-N1 eu-karyotic expression vector. After transfected the recombinant plasmid into THP-1 cells, the expression of IL-37 was detec-ted by RT-qPCR and Western blot. Results Double restriction enzyme digestion and gene sequencing showed that IL-37b gene was correctly inserted into the eukaryotic expression vector pEGFP-N1. RT-qPCR and Western blot showed that the IL-37 expression level was increased significantly (P<0. 01) after transfection in THP-1 cells. Conclusions We successful-ly constructed a novel anti-inflammatory cytokine IL-37 eukaryotic expression vector pEGFP-N1/IL-37b, which lays a foun-dation for further study on IL-37 functions and its association with related diseases.
