Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death
10.3969/j.issn.2095-4344.2016.29.015
- VernacularTitle:热缺血20 min猪心脏死亡供体心冠状动脉内皮功能的变化
- Author:
Xiaoke SUN
;
Weihua ZHANG
;
Guangqi QIN
;
Qiuming LIAO
;
Steen STIG
;
Chenhui QIAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(29):4362-4367
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE:To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS:Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups. RESULTS AND CONCLUSION:There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P>0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.
