Effects of CUEDC2 on inflammation and renal interstitial fibrosis in obstructive nephropathy

VernacularTitle:CUEDC2对梗阻性肾病小鼠肾组织炎症反应及间质纤维化的作用
Author: Meng SHEN ; Zhengmao LUO ; Hong ZHANG ; Yuanhang HUANG ; Lang LI ; Feng HE ; Junrong TONG
Publication Type:Journal Article
Keywords: CUEDC2; Collagen I; Inflammation factors; Renal interstitial fibrosis
From: The Journal of Practical Medicine 2016;32(12):1907-1911
CountryChina
Language:Chinese
Abstract: Objective To study the effects of CUEDC2 on renal interstitial fibrosis and inflammation response in rats with unilateral ureteral obstruction (UUO). Methods 30 Balb/c rats were randomly distributed into sham operation group(sham-vector),uuo operation group(uuo-vector) and CUEDC2 treatment group after uuo (uuo-cuedc2). Hematoxylin-eosin and Masson staining were used to measure renal pathology; Inflammation factors were quantified by ELISA; Immunohistochemistry was performed to measure the expression of CUEDC2;Protein expression of CUEDC2, Fibronectin, E-cadherin, Collagen I were detected by Western Blot. Results At 7 and 14d after operation, the area of interstitial fibrosis and expression of ICAM1,MCP1,IL1,IL8, Fibronectin and Collagen I in uuo-cuedc2 showed a marked decrease when compared to uuo-vector (p?0.05),the level of E-cadherin was significantly increased (P < 0.05). Conclusion CUEDC2 can inhibit renal interstitial fibrosis and decrease the expression of inflammation factors and Collagen deposition.