Bone marrow mesenchymal stem cell transplantation for rat cerebral infarction:recovery of neurological function and expression of synaptophysin
10.3969/j.issn.2095-4344.2016.28.012
- VernacularTitle:骨髓间充质干细胞移植脑梗死大鼠：神经功能恢复与突触素的表达
- Author:
Lvfang CHENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(28):4182-4188
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Synaptophysin plays an important role in the recovery of neural function after cerebral ischemia. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem cel transplantation on nervous function and expression of synaptophysin after cerebral infarction. METHODS:Total y 60 rats were equivalently randomized into four groups, including sham operation, control, model and stem cel treatment groups. Rats in the control, model and stem cel treatment groups were used for preparing cerebral infarction models, and the remaining underwent the sham operation. After 1 day of modeling, bone marrow mesenchymal stem cel s were transplanted into the rat lateral ventricle in the stem cel treatment group, and rats in the control group was given the injection of the same amount of PBS. After 1, 7 and 14 days of treatment, rat’s neurological function was scored on beam-walking test, rotarod test and screen test, and expression of synaptophysin was detected by RT-PCR and immunohistochemical assay. RESULTS AND CONCLUSION:At 7 and 14 days after treatment, the beam-walking test, rotarod test and screen test scores in the stem cell treatment group were significantly lower than those in the control and model groups (P<0.05), and the above scores showed no significant differences between the control group and model group (P>0.05). At 1 day after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cel s in the sham operation group were significantly higher than those in the other three groups (P<0.05);at 7 and 14 days after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the stem cell treatment group were significantly increased compared with the other three groups (P<0.05), and additionally, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the sham operation group were significantly lower than those in the model and control groups (P<0.05). These findings suggest that bone marrow mesenchymal stem cell transplantation can effectively promote the recovery of neurological function in cerebral infarction rats, and partially promote the formation of synaptophysin.
