Protective effect of bone marrow stromal stem cells against acute lung injury after hip fracture in aged rats with chronic obstructive pulmonary disease
10.3969/j.issn.2095-4344.2016.28.013
- VernacularTitle:骨髓基质干细胞对熏烟暴露老年大鼠髋部骨折后急性肺损伤的保护作用
- Author:
Guitong ZHANG
;
Jieyang FENG
;
Jia LIU
;
Yanjin ZHANG
;
Zimin XIANG
;
Fanxing MENG
;
Tiansheng SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(28):4189-4195
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The elderly hip fracture patients with chronic obstructive pulmonary disease (COPD) have a higher postoperative mortality than those only with hip fractures. In recent years, it has become an issue of concerns. Because the mechanism is unknown, however, there are no effective clinical interventions for these patients. OBJECTIVE:To observe the effect of bone marrow stromal stem cel s (BMSCs) on the level of pulmonary inflammation in aged rats with chronic obstructive pulmonary disease (COPD) after hip fractures. METHODS:Thirty male Sprague-Dawley rats, 12 months old, were exposed to smoking for 4 months and randomized into three groups, smoking, smoking+hip fracture+normal saline, smoking+hip fracture+BMSCs groups. Animal models of hip fracture were made in the latter two groups. Twenty-four hours after hip fracture, the lower lobe and peripheral blood samples were taken from al rats in the three groups, to evaluate the pathological changes of lung tissue and detect levels of inflammatory factors in the lung tissue and peripheral blood. RESULTS AND CONCLUSION:After closed hip fracture, aged COPD rats exhibited inflammatory cel infiltration, mucus secretion, airway stenosis or occlusion;the levels of interleukin-6, tumor necrosis factor-αand interleukin-10 in the lung tissue and peripheral blood were increased. After intravenous injection of BMSCs, the pathological changes of the lung tissue were reduced, and the levels of pro-inflammatory factors, tumor necrosis factor-αand interleukin-6, decreased, but the level of anti-inflammatory factor interleukin-10 further increased, which were significantly different from those in the normal saline group (P<0.05). These findings indicate that BMSCs can relieve acute lung injury in aged COPD rats with hip fractures.
