Effects of Bone Marrow Stromal Cells Transplantation Combined with Ultrashort Wave Therapy on the Expression of GFAP and ED-1 after Spinal Cord Injury in Rats
10.12007/j.issn.0258-4646.2016.08.002
- VernacularTitle:骨髓间充质干细胞联合超短波对大鼠脊髓损伤后早期GFAP和ED-1的影响
- Author:
Shi SUN
;
Yucen WAN
;
Lina ZHAO
;
Yanmei YIN
;
Yu LU
;
Zhiping FENG
;
Yuxin ZHOU
;
Zhiqiang ZHANG
;
Lixin ZHANG
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
bone marrow stromal cells;
ultrashort;
glial fibrillary acidic protein;
ED-1
- From:
Journal of China Medical University
2016;45(8):678-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of bone marrow stromal cells(BMSCs)transplantation combined with low dose ultrashort wave (USW)radiation on functional recovery and the expression of glial fibrillary acidic protein(GFAP)and ED?1 after spinal cord injury(SCI)in rats,and further discuss its action mechanism. Methods Female Sprague?Dawley rats(n=30)were randomly divided into 5 groups:sham?oper?ated,as well as control,USW,BMSCs,and USW+BMSCs that were subjected to spinal cord injury(SCI). Basso?Beattie?Bresnahan(BBB)tests were carried out before the operation and at 1 d,1 week,2 weeks,3 weeks,4 weeks after SCI. 4 weeks later,animals were sacrificed and tissues were collected to make paraffin section. Immunohistochemical staining was performed to observe the expression of GFAP and ED?1. Results 4 weeks after SCI,BBB scores were significantly higher in the USW and USW+BMSCs groups than in the control group(both P<0.001). No signifi?cant difference was observed between the BMSCs group and control group. On the expression of GFAP ,only USW+BMSCs group showed signifi?cantly decreased compared with the control group(P<0.05). All treatment groups exhibited lower ED?1 expression than the control group(all P<0.05). Conclusion Our results indicate that USW radiation alone can obviously improve neural functional recovery after SCI. The USW radi?ation and BMSCs transplantation treatment can reduce inflammation ,and USW radiation is more effective. The combination therapy did not show a synergistic action on promoting functional recovery ,but do have an effect on reducing the inflammatory response and glial scar formation.
