Characterization of UGT1a1, UGT1a6 and mGST1 at different stages of differentiation of mouse embryonic stem cells
10.3969/j.issn.2095-4344.2016.28.004
- VernacularTitle:小鼠胚胎干细胞不同分化阶段UGT1a1、UGT1a6和mGST1的表达特征
- Author:
Lingli XU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(28):4130-4135
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Until now, limited information has been neported on the characterization of udines 5-diphosphateglucuronosyl transferase (UGT) 1a1, UGT1a6, and microsomal glutathione S-transferase 1 (mGST1) during the differentiation of mouse embryonic stem cel s. OBJECTIVE:To observe the expression characteristics of UGT1a1, UGT1a6 and mGST1 at different stages of differentiation of mouse embryonic stem cel s. METHODS:Embryonic fibroblasts from Wistar rats were isolated and cultured as feeder cel s. Mouse embryonic stem cel s seeded onto these feeder cel s were induced to differentiate into hepatocytes. Subsequently, expression of UGT1a1, UGT1a6 and mGST1 was detected using western blot assay, and catalytic activity of mGST1 was determined by high performance liquid chromatography. RESULTS AND CONCLUSION:Increasing UGT1a1 expression was visible during the whole cel differentiation, while UGT1a6 exhibited no expression initial y but a higher level at 18 days of differentiation. mGST1 expression was visible at high level throughout the differentiation, but its expressive abundance was stil lower than that in the adult mouse stem cel s. At 18 days after the beginning of differentiation, the catalytic activity of mGST1 in microsomal liver tissue was 7.65μmol/(min?g). Taken together, stabilized UGT1a1, increased UGT1a6 and little mGST1 expression are confirmed in the differentiation of embryonic stem cel s into hepatocytes.
