Effects of propofol against LPS-induced inflammation injury of Ana-1 cells
- VernacularTitle:丙泊酚对脂多糖引起的 Ana-1巨噬细胞炎症损伤的影响
- Author:
Xuedong WANG
;
Min LI
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Propofol;
Ana-1 cell;
Inflammation injury
- From:
The Journal of Clinical Anesthesiology
2016;32(6):590-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of propofol on lipopolysaccharide (LPS)-induced Ana-1 cell damage.Methods The LPS-induced inflammation damage of Ana-1 cells was es-tablished as a model.Ana-1 cells were divided into control group(group C),LPS group(group L), LPS+propofol 10μmol/L group(group LP1),LPS+propofol 20μmol/L group(group LP2),LPS+propofol 40 μmol/L group(group LP3),and LPS+propofol 80 μmol/L group(group LP4),respec-tively.Cell survival rate was assessed using MTT,interleukin 1βand 6,and tumor necrosis factors (TNF-α)were determined.Results Survival rate of Ana-1 cells in group L was lower than that in group C,while it was significantly improved in group LP1,LP2,LP3,LP4 in a dose-dependent man-ner (P <0.01);IL-1β,IL-6,TNF-αrelease was significantly higher in group L than in group C;IL-1β,IL-6,TNF-αrelease significantly reduced in group LP1,LP2,LP3,LP4 (P <0.01)in a dose-dependent manner (P <0.01).Within the selected dose range in this experiment,group LP4 had the best protection effect.Conclusion Propofol can reduce inflammation damage of Ana-1 cells caused by LPS.
