Protective effect of ligustrazine on lipopolysaccharide induced vascular hyperpermeability
10.3969/j.issn.1006-5725.2016.12.008
- VernacularTitle:川芎嗪对脂多糖诱导的血管通透性增加的保护作用
- Author:
Juxiang HE
;
Yong ZHANG
;
Yan LI
;
Junfeng HU
;
Yumei SUN
;
Xiaofang HU
- Publication Type:Journal Article
- Keywords:
Ligustrazine;
lipopolysaccharide (LPS);
endotoxin;
Rho/ROCK signaling pathway
- From:
The Journal of Practical Medicine
2016;32(12):1920-1923
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether ligustrazine can reduce endotoxemia and inhibit the increase of lipopolysaccharide (LPS)-induced vascular permeability by suppressing Rho/ROCK signaling pathway in guinea pigs. Methods Eighteen of white male healthy guinea pigs were divided into three groups randomly and pretreated with intravenous injection of ligustrazine(3 or 6 mg/kg) or saline. 30 min later, LPS (100, 300, 1 000 μg/0.1 mL/site) and saline (0.1 mL/site) were administered intracutaneously at the dorsum of guinea pigs. Vascular permeability was determined by leakage area and absorbance of 610 nm (OD610) of Evans Blue dye after intracutaneous injection of LPS (100-1 000 μg/site). Results Dye leakage in the skin began to increase at 5 min, and revealed significant increase 2 h after LPS injection. Significant differences in the dye leakage area(F = 5.77, P < 0.05) and the OD610 (F = 7.736, P < 0.05) induced by 100, 300 and 1,000 ug/site of LPS administration were demonstrated between the control and ligustrazine groups (3 or 6 mg/kg). Conclusion Ligustrazine has protective effect on the hypermeability of endothelial cells induced by lipopolysaccharide, and inhibition of Rho/ROCK signaling pathway is a possible mechanism.
