Effects of beraprost sodium on apoptosis in cultured mouse podocyte induced by angiotensin Ⅱ
10.3969/j.issn.1006-5725.2016.12.009
- VernacularTitle:贝前列素钠对血管紧张素Ⅱ诱导小鼠足细胞凋亡的影响
- Author:
Ying LIN
;
Yi QIAN
;
Yaoming XUE
;
Qian ZHANG
- Publication Type:Journal Article
- Keywords:
Beraprost sodium;
AngiotensinⅡ;
Podocyte;
peroxisome proliferator activated receptorδ
- From:
The Journal of Practical Medicine
2016;32(12):1923-1926
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of beraprost sodium (BPS) on angiotensin Ⅱ(AngⅡ)-induced podocyte apoptosis and the relationship between BPS and peroxisome proliferator activated receptor δ(PPARδ). Methods Differentiated mouse podocytes were exposed to AngⅡ, further treated with BPS (or GSK0660) for 24 h. Podocyte apoptosis was detected by flow cytometry and AnnexinV-FITC staining. Bax , Bcl-2 and PPARδ mRNA were assessed by RT-PCR. Results AngⅡ promoted podocyte apoptosis and Bax mRNA expression significantly, down-regulated Bcl-2 and inhibited PPARδ mRNA (P < 0.05). Conversely, treatment with BPS reduced AngⅡ-induced podocyte apoptosis and Bax mRNA expression and promoted Bcl-s expression significantly resulting in an increase of PPARδ mRNA expression (P < 0.05). With the inhibition of PPARδ, podocyte apoptosis and Bax mRNA expression increased while Bcl-2 mRNA expression reduced. Conclusion BPS can effectively reduce AngⅡ-induced podocyte apoptosis , which is associated with the activation of PPARδand regulation of Bcl-2/Bax mRNA expression.
