Signaling pathways analysis of effects of BSP on RAW264.7 cells proliferation and differentiation

VernacularTitle:骨唾液酸蛋白对破骨细胞前体RAW264．7增殖、分化作用的信号通路
Author: Yu SHI ; Yan ZHANG ; Lisha LIN ; Sinian LIU ; Yi LI ; Chao HAN ; Ling LI
Publication Type:Journal Article
Keywords: BSP; RAW264.7; Proliferation; Differentiation; signaling pathways
From: The Journal of Practical Medicine 2016;32(11):1734-1737
CountryChina
Language:Chinese
Abstract: Objective To explore the key signaling pathways and molecules of bone sialoprotein (BSP) to promote proliferation and differentiation of preosteoclasts RAW264.7. Methods RAW264.7 cells were treated with BSP and the inhibitors of signaling pathway molecules. MTS and TRAP staining kits were used to evaluate the effects of proliferation and differentiation. The activity assay kits of Calcineurin, AKT, JNK, ERK and p38 were used to detect their activities. Results Inhibiting ERK and p38 can inhibit cell proliferation induced by BSP significantly, while inhibiting AKT, Calcineurin and PI3K can reduce the role of promoting the differentiation by BSP. With increasing treatment time of BSP, the activity of Calcineurin in RAW264.7 cells increased. Furthermore, the activity of AKT was maximum at 48 h after treated with BSP, while the activity of JNK, ERK and p38 were maximum at 24 h after treated with BSP. Conclusions BSP mainly regulates the ERK and p38 of MAPK pathway to promote RAW264.7 cell proliferation, and regulates AKT, PI3K and Calcineurin pathways to promote RAW264.7 cell differentiation.