Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance
10.13481/j.1671-587x.20160409
- VernacularTitle:MT01对牙龈卟啉单胞菌感染成骨细胞 MG63中Ⅰ型胶原mRNA 表达的促进作用及其意义
- Author:
Yin LIU
;
Yuqin SHEN
;
Han GAO
;
Hongbo FEI
;
Tianqi HU
;
Yangyang LI
;
Zhongyi GU
;
Chongtao LIN
- Publication Type:Journal Article
- Keywords:
specific sequence oligodeoxynucleotide MT01;
Porphyromonas gingivalis;
human osteoblast cell;
collagen Ⅰ
- From:
Journal of Jilin University(Medicine Edition)
2016;42(4):681-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of MT01 on the differentiation of osteoblasts under infected condition through determining the expression level of collagen Ⅰ (Col Ⅰ )mRNA in MG63 cells treated with Porphyromonas gingivalis (Pg).Methods:The cultured MG63 cells were divided into blank control,MT01,Pg, and MT01+Pg groups.MT01 at a concentration of 1 mg·L-1 was added into the MG63 cells,and the cells were incubated for 3 h.The cells treated with PBS (1 mg·L-1 )were used as control group.Then Pg (MOI=100∶1) was added.Real-time PCR was used to detect the expression levels of ColⅠ mRNA in MG63 cells at 2,4,6,8, 12 and 24 h after incubation.Results:Compared with blank control group,the levles of ColⅠ mRNA in the MG63 cells in MT01 and MT01 + Pg groups had no significant changes at 2 and 4 h (P > 0.05);the Col Ⅰ mRNA expression levels in MT01 group at 8,12 and 24 h were increased (P < 0.05 or P < 0.01).Compared with Pg group,the expression levels of ColⅠ mRNA in MT01+ Pg at 2 and 4 h were decreased,but the expression levels of ColⅠ mRNA were increased at 6,8,12 and 24 h (P <0.05 or P <0.01).Conclusion:MT01 can up-regulate the expression level of Col Ⅰ mRNA in the infected MG63 cells; MT01 could promot the differentiation of osteoblasts under infected condition.
