Application of 9.4 T 1 H-MR spectroscopy in determination of lactate in brain of mice with early acute hypoxia-ischeia injury and its significance
10.13481/j.1671-587x.20160411
- VernacularTitle:9.4T 高场磁共振频谱在急性缺血缺氧损伤早期小鼠脑代谢物乳酸测定中的应用及其意义
- Author:
Yunping ZHANG
;
Yangkang LI
;
Chenyang LIU
;
Jiahui ZHU
;
Renhua WU
- Publication Type:Journal Article
- Keywords:
acute cerebral ischemia;
lactate;
cerebral metabolites;
proton magnetic resonance spectroscopy
- From:
Journal of Jilin University(Medicine Edition)
2016;42(4):690-693
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the lactate metabolism in brain tissue of the mice with early acute hypoxia-ischemia injury,and to provide data support for 9.4T 1 H-NMR spectroscopy in detecting the lactate level clinically.Methods:Eighty Kunming mice were randomly divided into sixteen groups (0 s,20 s,40 s,60 s,2 min,4 min, 6 min,8 min, 10 min, 12 min, 14 min, 16 min, 18 min,and 20 min)according to the duration of hypoxia-ischemia (n=5).The changes of lactate levels were detected by 9.4T 1 H-NMR spectroscopy. Results:After the initiation of hypoxia-ischemia injury,the lactate level began to increase rapidly to the highest value of (6.89 ± 0.34)μmol·g-1 at 20 s,then started to decline quickly from 40 s to 2 min,and eventually decreased to a stable level of (4.85±0.36)μmol·g-1 until 6 min.Compared with control group,the levels of lactate in brain tissue of the mice in hypoxic-ischemic groups were increased (P <0.01).Conclusion:40 s of acute hypoxia-ischemia may be the lactate cerebral neuron threshold during the anaerobic glycolysis. 9.4T1 H-MRS can provide the exact time window for detecting the lactate metabolism.
