Induction effect of NPPB on apoptosis of human glioma SHG-44 cells and its mechanism
10.13481/j.1671-587x.20160401
- VernacularTitle:NPPB 对人脑胶质瘤 SHG-44细胞凋亡的诱导作用及其机制
- Author:
Jing TIAN
;
Ling QI
;
Pengyan JI
;
Nan SHEN
;
Wanli CUI
;
Chunyan WANG
- Publication Type:Journal Article
- Keywords:
chloride channel;
glioma;
cell proliferation;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2016;42(4):637-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the induction effect of NPPB,a chloride channel blocker,on the apoptosis of human glioma SHG-44 cells,and to explore its mechanism. Methods:The SHG-44 cells were cultured in vitro and divided into control group and NPPB groups (50,100,200 μmol· L-1 ).The cell viability was detected by MTT assay.The apoptotic rates were detected by flow cytometry.The expression levels of Bax, Bcl-2 and caspase-3 were detected by immunohistochemical analysis and Western blotting method.Results:Compared with control group,the cell viabilities of SHG-44 cells in 100 and 200 μmol·L-1 NPPB groups after treated for 24 and 48 h were decreased significantly (P < 0.01).The results of flow cytometry showed that the apoptotic rates of SHG-44 cells in 100 and 200 μmol·L-1 NPPB groups were 24.64% and 41.85%,and they were higher than that in control group (4.17%) (P <0. 01).The immunohistochemical analysis and Western blotting results showed that the expression levels of caspase-3 and Bax proteins in SHG-44 cells in 100 μmol · L-1 NPPB group were increased (P < 0.05 or P < 0. 01 ), and the expression level of Bcl-2 protein was decreased (P < 0.05 ). Conclusion:NPPB could induce the apoptosis of human glioma SHG-44 cells by the down-regulation of the expression of Bcl-2 and the up-regulation of the expression of Bax,and the activation of caspase-3.
