1 H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats
10.11895/j.issn.0253-3820.150934
- VernacularTitle:基于1HNMR给药赭石后大鼠尿液的代谢组学分析
- Author:
Ying WEI
;
Chuanling WAN
;
Rong XUE
;
Xiaojing LI
;
Wenjun ZHANG
;
Fengkui PEI
- Publication Type:Journal Article
- Keywords:
Nuclear magnetic resonance;
Metabonomics;
Principal component analysis;
Partial least squares-discriminant analysis;
Haematitum
- From:
Chinese Journal of Analytical Chemistry
2016;44(6):857-863
- CountryChina
- Language:Chinese
-
Abstract:
Wistar rats were intragastrically administered with different doses (2, 5 and 10 g / kg body weight) of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group.
