Analysis of ISG15-Modified Proteins from A549 Cells in Response to Influenza Virus Infection by Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.151011
- VernacularTitle:液相色谱-质谱联用分析流感病毒感染引起宿主蛋白类泛素化修饰
- Author:
Qisheng PENG
;
Guangpu LI
;
Wanchun SUN
;
Jingbo YANG
;
Guihua QUAN
;
Ning LIU
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Ubiquitin-like protein;
Interferon stimulated gene 15 kDa protein;
Liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2016;44(6):850-856
- CountryChina
- Language:Chinese
-
Abstract:
Interferon stimulated gene 15 kDa protein (ISG15) is the first ubiquitin-like protein identified, which plays vital roles in a variety of fields including viral infection and immunological regulation. In this study, liquid chromatography-tandem mass spectrometry was used to analyze ISG15-modified proteins in A549 cells in response to infection by influenza virus, which was enriched by immunoprecipitation. A total of 22 cellular host proteins were identified in A549 cells infected by influenza virus, including ubiquitin-like ISG15 protein, cyclin-T1, heat shock protein 71 kDa, caldesmon, eukaryotic translation initiation factor, and so on. Besides, non-structural protein (NS1) from influenza virus was also identified. Among the 22 host proteins identified, 6 proteins were also identified in the control non-infected A549 cells, including annexin A1, fructose-bisphosphate aldolase A, ATP synthase subunit g, enolase, actin, and tubulin. Bioinformatics analysis revealed that the identified ISG15-modified host proteins induced by influenza virus infection could be classified into 9 protein classes: chaperone, oxidoreductase, enzyme modulator, transferase, nucleic acid binding, transcription factor, kinase, cytoskeletal protein, and structural protein. This study provided a specific and effective tool for analyzing ISG15-modified proteins in proteome level.
