Blockade of CLC-3 chloride channel inhibited the viability and invasion of colorectal cancer cells
10.3969/j.issn.1000-8179.2016.09.065
- VernacularTitle:阻断CLC-3氯通道对结直肠癌细胞株生存率和侵袭转移能力的影响及其分子机制
- Author:
Yanping WANG
;
Linsong JI
;
Hongwei FAN
;
Xiaohui XIANG
;
Wei XU
- Publication Type:Journal Article
- Keywords:
CLC-3;
colorectal cancer;
Wnt/β-catenin signaling pathway;
cell viability;
invasion
- From:
Chinese Journal of Clinical Oncology
2016;43(9):361-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the expression of CLC-3 in colorectal tissues and the effect of CLC-3 on the viability and invasion of colorectal cancer (CRC) SW480 and SW620 cells. Methods:The mRNA levels of CLC-3 in CRC cell lines were determined by RT-PCR. CLC-3 expression was inhibited by adding DIDS or NPPB to the CRC cells. Subsequently, cell viability and invasion were assessed by CCK-8 assay and Transwell assay, respectively. In addition, the effects of DIDS and NPPB on the Wnt orβ-catenin signaling pathways were de-termined by Western blot analysis. Results:The mRNA level of CLC-3 was remarkably increased in the CRC tissues compared with that in normal colorectal tissues (P<0.05) and was positively correlated with the T stage of CRC. The blockade of CLC-3 inhibited the viability and invasion of CRC cells (P<0.05). The expression ofβ-catenin, C-myc, cyclin D1, Ki-67, and survivin were evidently reduced by the in-hibition of CLC-3 (P<0.05). Conclusion:The inhibition of CLC-3 decreases the cell viability and invasion of CRC cells by reducing the ex-pression of the proteins related to the Wnt orβ-catenin signaling pathway.
